| 1. | Expression of e2 gene of classical swine fever virus in insect cell
中的表达及其免疫活性的初步研究 |
| 2. | Expression and purification of erns gene of classical swine fever virus in e . coli
2基因在家蚕杆状病毒表达系统中的融合表达 |
| 3. | Co - expression of classical swine fever virus e2 gene with bovine herpesvirus 1 ul49 gene
2基因在昆虫细胞中的分泌表达及活性检测 |
| 4. | Studies on construction and immunity enhance of double expression plasmids of classical swine fever virus e
2基因真核双表达载体的构建及其免疫增强作用的研究 |
| 5. | Studies on enhaning effect of alfalfa polysaccharide on the immune response of lapinized chinese strain of classical swine fever virus
苜蓿多糖对猪瘟兔化弱毒疫苗免疫应答强化作用的研究 |
| 6. | Molecular cloning and highly effective expression of c - terminally truncated ns3 gene of classical swine fever virus chinese strain in escherichia coli
3丝氨酸蛋白酶功能区基因的克隆及其在大肠杆菌中的高效表达 |
| 7. | The glycoprotein eo of classical swine fever virus ( csfv ) , besides being an envelope protein , possesses knase activity , which is pertinent to viral persistent infection in the host
猪瘟病毒( classicalswinefevervirus , csfv ) eo糖蛋白既是包膜蛋白，又是一种核酸酶，其活性对病毒在宿主体内的持续感染有直接关系。 |
| 8. | In this experiment , to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all , which had been prepared in previous experiment , the mcab all was raised in mice and followed by purification , the concentration of protein was assayed by using the bca protein assay kit
本室利用e2基因疫苗制备了多株单抗，为e2的抗原表位研究提供了条件，我们以噬菌体随机12肽库分析鉴定了猪瘟病毒e2蛋白的抗原表位，为深入研究猪瘟病毒的抗原结构、制备更有效的诊断试剂和疫苗提供更多理论依据。 |
| 9. | In this study , the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced . the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues
研究采用反转录?聚合酶链式反应( rt ? pcr )技术对两株分别从柳州( gxlz )和南宁( gxnn )分离的广西流行猪瘟病毒( classicalswinefevervirus ， csfv )进行e _ 2全基因的扩增、克隆和测序。扩增片段长度为1090bp ，编码364个氨基酸残基。 |
| 10. | According to the published nucleotide sequences of genome of classical swine fever virus ( csfv ) strains shimen , hclv and paderborn , 11 pairs of specific primers were designed and synthesized , eleven fragments had been successfully amplified from csfv gxwz02 strain by rt - pcr . these amplified fragments were cloned into pgem - t or pmd18 - t and sequenced
根据已发表的猪瘟病毒( classicalswinefevervirus ， csfv ) shimen株、 hclv株和paderborn株的全基因组序列，设计并合成11对引物，应用rt - pcr技术，成功地分11个片段扩增了csfv广西流行毒株gxwz02的全基因组，并将这11个基因片段克隆到pgem - t和pmd18 - t载体上，测定其核苷酸序列。 |
